Study Synopsis

DNA methyltransferase I (DNMT1) plays a key role in establishing and maintaining patterns of DNA methylation. Dysregulation of DNA methylation is associated with different kinds of cancer. The goal of this work was to compare the proteomes of cells with a hypomorphic mutation of DNMT1 to those that had normal DNMT1 (The hypomorphic DNMT1 mutation encodes a DNMT1 protein with a truncated N-terminus, and it is expressed at lower levels).

Shotgun mass-spectrometry proteomics was used to profile proteins in the nuclei of HCT116 (a colorectal cancer cell line) that either had normal, full-length DNMT1 or had the hypomorhic allele of DNMT1

The main finding of this study (Link to PubMed) was that proteins involved in the Epithelial-Mesenchymal transition (a key process in tumour metastasis) are increased in expression in the DNMT1 hypomorphic cells, suggesting that DNMT1 has a role in regulating the Epithelial-Mesenchymal transition.

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MS/MS data Files (peak lists)

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Raw Data

(Note that condensed MGF files are provided here, since the complete files are very large)

Searched/Quantification Results

Taxonomy: Homo sapiens


Bowler et al. Deep proteomic analysis of Dnmt1 mutant/hypomorphic colorectal cancer cells reveals dys-regulation of Epithelial-Mesenchymal Transition and subcellular relocalization of Beta-Catenin. Epigenetics. 2019 Aug 26:1-15 (PubMed)