Study Synopsis

This study used shotgun proteomics to study the proteomes of mouse embryonic stem cell sub-cellular compartments. Whole embryonic stem cell lysates were fractionated into nuclear and cytoplasmic components using low-speed centrifugation. We then used 1-Dimensional gel-based fractionation to separate the nuclear and cytoplasmic samples into 5 fractions each (Song et al, 2012). The data provided here correspond to one each of these fractions for the nuclear (Nuc) sample and the cytoplasmic (Cyto) samples.

Tasks & Questions

Search the Nuclear and Cytosolic MS/MS spectra using Tandem search engine
Compare the sets of proteins identified for the Nuclear and Cytosolic samples using GOrilla
Do the identified Gene Ontology terms suggest enrichment of Nuclear and Cytosolic proteins in the Nuc and Cyto samples?

Data

MS/MS data Files (peak lists)

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Raw Data

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Taxonomy: Mus musculus

References

Song J, Saha S, Gokulrangan G, Tesar PJ, Ewing RM. DNA and chromatin modification networks distinguish stem cell pluripotent ground states. Molecular & cellular proteomics : MCP. 2012; 11(10):1036-47 (PubMed).